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ISIS Report 07/05/04
GM Food & Feed Not Fit for "Man or Beast"
Dr. Mae-Wan Ho and Prof. Joe Cummins review some
of the scientific evidence behind a series of recent scandals involving the
safety of GM food and feed. They expose fatal flaws in the regulatory process
and highlight how Europe is in danger of approving GM varieties that are genetically
unstable and hence illegal as well as unsafe. They demand a full enquiry into
the abuse of science that has allowed GM crops not fit for human or animal consumption
to enter our food chain.
Based on a paper presented at an ISP Briefing to Parliament, House
of Commons, 29 April 2004.
Mae-Wan Ho and Joe Cummins
Institute of Science in Society www.i-sis.org.uk and
Independent Science Panel www.indsp.org
Latest incidents to cast doubt on the safety of GM food
The European Food Safety Authority (EFSA) has given Monsanto's GM maize Mon863,
containing the biopesticide Cry3Bb1 against the corn rootworm, a positive assessment.
However, French newspaper Le Monde  has seen secret documents revealing
health impacts of the GM maize, described as "very disturbing" by scientists
of the French commission for genetic engineering (CBG), including kidney malformations
and increases in white blood cells in male rats and high blood sugar and reduced
immature red blood cells in female rats.
Last year, up to 100 villagers in the south of the Philippines living near
GM maize plots suffered debilitating illnesses when the GM maize came into flower
. Prof. Terje Traavik of the Norwegian Institute of Gene Ecology in Tromsø
found antibodies to Cry1Ab produced by the GM maize against the corn borer in
the blood of 39 villagers . The maize variety was Dekalb 818 YG, a hybrid
between Monsanto's Mon 810 and a locally adapted variety (Dekalb 818).
Report has come in of the same illnesses recurring this year .
Bt toxins known to be harmful
The Cry proteins, dozens of them, are also called Bt toxins because they are
produced by different strains of the soil bacterium Bacillus thuringiensis
[5, 6]. Reports in the scientific literature have documented that bacterial
spores of B. thuringiensis, containing a mixture of different toxins,
can cause allergic reactions in farm workers; that some toxins are immunogenic
in animals, Cry1Ac in particular, has been identified as a potent immunogen,
as potent as cholera toxin; that cells in the lining of the small intestine
in rats have proteins that bind to the toxins , and further, Cry1Ab protein
is 92% indigestible in pigs .
Regulatory sham over Bt crops
The findings on Bt toxins have been completely ignored in a regulatory process
that can only be described as a sham .
Worse still, Bt genes in crops are synthetic or hybrid constructions, with
important changes from the naturally occurring bacterial genes. Yet, toxicity
tests are routinely done using the natural toxins, and not the toxin produced
in the GM crop plants, with the result that the Bt toxins in GM crops
are almost completely unknown and untested for toxicity [5, 6].
There’s evidence that the natural toxin is not the same as, or “substantially
equivalent” to, the GM toxin. Green lacewings suffer significantly reduced
survival and delayed development when fed an insect pest (lepidopteran) that
has eaten GM maize containing the Bt toxin Cry1Ab, but not when fed the same
pest treated with much higher levels of the natural toxin [9, 10]. This is an
extremely important effect passed on through the food chain; and has been documented
in several laboratories. Unfortunately, the researchers misrepresented the results
to mean that Cry1Ab does not harm beneficial insect predators .
All GM genes differ from natural genes
All foreign genes inserted into GM organisms are different from their natural
counterparts. The minimum construct consists of a promoter, a gene-switch that
says to the cell, "copy the following message (the gene or coding sequence)
for making a protein", and another signal, the terminator, to
say, "stop here, end of message". All three parts are often from
different sources. The gene itself could also be a composite of different DNA,
often made artificially in the laboratory .
It is generally not easy to get the foreign gene to work, so a very aggressive
promoter is needed, literally to force the cell to make the protein.
The cauliflower mosaic virus (CaMV) 35S promoter is the most popular one used,
and is often accompanied by other 'boosters' from a variety of sources.
For example, Mon 863 maize is described on the AGBIOS Database as follows :
"The introduced DNA contained the modified cry3Bb1 gene from B. thuringiensis
subsp. kumamotoensis under the control of the 4-AS1 promoter (CaMV 35S
promoter with 4 repeats of an activating sequence), plus the 5' untranslated
leader sequence of the wheat chlorophyll a/b binding protein (wt CAB leader)
and the rice actin intron. The transcription termination sequence was provided
from the 3' untranslated region of the wheat 17.3 kD heat shock protein (tahsp17).
The modified cry3Bb1 gene encodes a protein of 653 amino acids whose amino acid
sequence differs from that of the wild-type protein by the addition of an alanine
residue at position 2 and by seven amino acid changes."
There are thus 9 bits of DNA from different sources including the coding sequence,
which has been quite substantially altered from the natural gene.
The GM process is unreliable and uncontrollable
That's not all. The artificial constructs are further spliced into gene carriers
or vectors, and introduced into cells by invasive methods that result in random
integration into the genome, giving rise to unpredictable, random effects, including
gross abnormalities in animals and further unexpected toxins and allergens in
food crops .
A transgenic line is essentially regenerated from a single cell in which specific
GM DNA integration occurred. Each event will give rise to a different line.
In other words, there is no possibility for quality control. This problem is
compounded by the overwhelming instability of transgenic lines, because the
artificial constructs cobbled together from DNA of different sources tend to
have weak joints, especially if they include elements like the CaMV 35S promoter,
which is known to have a fragmentation or recombination hotspot (see later).
Transgenic lines are overwhelmingly unstable
We have referred to the instability of transgenic lines as the "best kept open
secret", because everybody has known about it for years, but agree to say nothing,
while regulators turned a blind eye .
(Claims of genetic stability based on the failure to depart from Mendelian
ratios have been widely accepted as evidence of Mendelian inheritance, i.e.,
a sign of genetic stability. But such claims are bogus for a number of reasons.
First, a 'Mendelian ratio' refers to the proportion of different classes of
offspring predicted from a cross involving different lines. It depends on assuming
that Mendelian inheritance is true; so in order to depart from a particular
ratio, a sufficiently large number of offspring are needed to obtain the required
level of significance (at 5%). Consequently, a failure to depart from the predicted
Mendelian ratio does not prove Mendelian inheritance. On the contrary, the real
inheritance may be non-Mendelian (a sign of genetic instability), but
an insufficient number of offspring has been produced for the statistical test
to reach the required level of significance.
More importantly, the precise Mendelian ratio to use in each case depends on
the genotype of the parents, and this needs to be independently ascertained,
but is almost never done. This makes nonsense of the predicted ratio.
Indeed, the Mendelian ratio used is always the one that most closely matches
the result obtained!
One of us had argued this very point at a public hearing on T25 maize in the
UK, and got the representative from the company Aventis to concede that Mendelian
ratios are not evidence of stability .)
Instead, we have been pressing, both in international biosafety conferences
and in print, for "event specific" molecular characterisation of the structure
of the insert(s) and their position(s) in the genome in successive generations,
as the only legitimate proof that the transgenic line is stable [14, 15]. This
requirement was finally written into the 2001 European Directive (2001/18/EC)
on the deliberate release of GMOs into the environment.
But it was not until last year that French government scientists checked the
transgenic inserts of five transgenic lines: Monsanto's Mon810 maize, Roundup
Ready soya, GA21 maize, Bayer's T25 maize and Syngenta's Bt 176 maize; and in
every case, the transgenic insert(s) had rearranged, not just from the construct
used, but since characterised by the company .
The results revealed that,
- All GM inserts had rearranged from the structure provided by the company
- Many of the breakpoints for rearrangement involve the CaMV 35S promoter,
as can be predicted from its known recombination hotspot
- Scrambling of the genome occurred at the site of insertion
- GM inserts appear to show a preference for mobile genetic elements (retrotransposons)
The last feature is particularly important, as retrotransposons contain strong
promoters that could alter gene expression, and also increase the chances that
the inserts will move again, resulting in further genome scrambling and horizontal
The French scientists presented their results in a poster at a conference
with the title: “Characterisation of commercial GMO inserts: a source
of useful material to study genome fluidity”. Genome fluidity underlies
the paradigm shift in genetics that makes genetic modification both futile and
Belgian government scientists carried out another study, confirming the instability
of the transgenic lines analysed by the French, and found that at least one
other transgenic line, Syngenta’s Bt 11 maize, had also rearranged, and
that it was contaminated with Bt176 .
In the case of other transgenic lines studied, it was unclear whether the company
has been allowed to submit new data since its first application for approval,
which would be irregular, to say the least.
For Roundup Ready soya GTS 40-3-2, for example, the French study found clear
evidence that the GM insert was unstable and had undergone rearrangement. The
Belgian study merely referred to the UK’s Advisory Committee for Novel
Foods and Processes (ACNFP) website, where it appears that the ACNFP had allowed
Monsanto to submit new data in 2000, and again in 2002, presumably to ‘correct’
its ‘error’ in the original dossier.
Transgenic instability is a key safety issue
There were small and large discrepancies between the French and Belgian studies,
which suggest that the transgenic lines were not only unstable but also non-uniform.
Either one of those should make the transgenic lines illegal for Europe.
There is every sign, however, that the European Commission will fudge this to
lift the de facto moratorium, which will be a criminal offence in our opinion,
as it will subject all European citizens to serious health risks.
Transgenic instability is a key safety issue. A GM variety that has changed
its identity since characterised by the company, invalidates any safety tests
or assessments that may have been done. It also makes it impossible to identify
the GM variety for post-release monitoring, for implementing remedial action
in case of harm and for assigning liability
Event specific characterisation of the GM inserts has only just begun. It is
not clear how many of the GM varieties currently pending approval in Europe
have been analysed (see Box 1).
It is also not legitimate to draw conclusions about the hybrids from data on
parental GM lines. We have pointed out , for example, in the case of NK603xMon810,
that both parental lines have rearranged, but no analyses were carried out on
the hybrid and seeds set by the hybrid, where further recombinations are expected
between the constructs, as they possess similar sequences that are recombination
hotspots (see later): CaMV 35S promoter with enhancer (e35S) and the hsp70 intron.
There can be no approval of any GM variety or hybrid for import, either for
growing or for food and processing unless and until event-specific analysis
has been carried out and the GM variety/hybrid proven to be stable.
Some GMOs pending approval in
||Draft decision to authorise**
||EFSA favourable opinion***
||EFSA favourable opinion
||EFSA favourable opinion
||insect & glyph. res.
||No decision from EFSA
||Belgian approval (but denied
||Positive assess. UK ACRE
||insect & glufo. res.
||Positive assess. Netherlands
||Consent from UK
* For import and/or use as food and/or feed and/or processing, not for
**Ministers of European countries failed to reach agreement on Bt11 for
food use, which is closest to final approval; the European Commission
will now have to decide.
***NK603 was rejected for animal feed and food use by EU member states;
the dossier now goes to the European Council of Ministers.
Major uncertainties over the safety of the GM process
Let us look at the rest of the evidence in brief; apart from the two incidents
- Between 2001 and 2002, twelve dairy cows died on a farm in Hesse, Germany,
after eating Syngenta’s Bt176 GM maize, and others in the herd had
to be slaughtered on account of mysterious illnesses . To-date, there
has been no detailed autopsy reports available, even though the company
claims the deaths and illnesses were unrelated to Bt176. Nevertheless the
Spanish Food Safety Authority has just withdrawn authorisation for Bt176
cultivation in Spain  after it had occupied almost all of the 20 000
hectares of GM maize grown in Spain since 1998 . The decision was taken
following an EFSA recommendation that GMOs containing antibiotic resistance
marker genes such as that found in Bt 176, be restricted to field trials.
- Arpad Pusztai and colleagues found that GM potatoes with snowdrop lectin
adversely affected every organ system of young rats, and the stomach and
small intestine lining grew up to twice the thickness of controls .
- Scientists in Egypt found similar results in the gastrointestinal tract
of mice fed GM potato with Bt toxin .
- US Food and Drug Administration had data since the early 1990s showing
that rats fed GM tomatoes with antisense gene to delay ripening developed
small holes in their stomach .
- Aventis (now Bayer) found 100% increase in deaths of broiler chickens
fed glufosinate-tolerant GM maize T25 compared to controls .
- Numerous anecdotes from farmers and others indicating that livestock,
wildlife and lab animals avoid GM feed, and fail to thrive or die when forced
to eat it [26, 27].
Different species of GM food or feed with different GM genes have caused problems
in many species of animals. You don't have to be a scientific genius to suspect
that there is something wrong with the GM process itself or the GM insert.
All of the GM inserts involved contain the CaMV35S promoter that we have warned
against since 1999 [28-31]. This promoter not only has a fragmentation hotspot
making transgenic lines extra unstable, it substitutes for the promoter of a
wide range of plant and animal viruses, and is also active in animal cells including
It is high time we ban all environmental releases of GM crops
to make way for non-GM sustainable agriculture .
The greatest obstacle to a safe and sustainable future is a corrupt
and corrupted science that operates on what can only be described as the anti-precautionary
principle. There must now be a thorough enquiry into the safety of GM food and
feed, and the systematic abuse of science that has allowed GM food and feed
to be approved, which had all the signs of being unsafe.
- "French experts very disturbed by health effects of Monsanto GM corn"
GMWatch www.gmwatch.org 23 April 2004
- "Filipino islanders blame GM crop for mystery sickness. Monsanto denies
scientist's claim that maize may have caused 100 villagers to fall ill"
John Aglionby in Kalyong, southern Philippines, The Guardian, Wednesday
3 March 3, 2004 http://www.guardian.co.uk/gmdebate/Story/0,2763,1160789,00.html
- Traavik, T. Lecture to Special Biosafety Genok and TWN Seminar, 22 February,
Kuala Lumpur, and personal communication.
- "Despite ban, agriculturists can't stop farmers from planting Bt corn",
Allen Estabillo, Minda News 23 April 2004 http://www.mindanews.com/2004/04/23nws-btcorn.html
- Cummins J. Regulatory sham over Bt-crops. ISIS report 1 December 2003;
also Science in Society 2004,
- Cummins J. Bt toxins in genetically modified crops: regulation by deceit.
Science in Society 2004, 22 (in press).
- Vázquez-Padrón RI, Gonzáles-Cabrera J, Garcia-Tovar
C, Neri-Bazan L, Lopéz-Revilla R, Hernández M, Moreno-Fierro
L and de la Riva GA. CrylAc protoxin from Bacillus thringiensis sp. kurstaki
HD73 binds to surface proteins in the mouse small intestine. Biochem
Biophys Res Commun 2000, 271, 54-8; Ho MW. Bt toxin binds to mouse intestine.
Science in Society 2004, 21, 7.
- Ho MW. Transgenic DNA & Bt toxin survive digestion. Science in Society
2004, 21, 11; Chowdhury EH, Kuribara H, Hino A, Sultana P, Mikami O, Shimada
N, Guruge KS, Saito M, Nakajima Y. Detection of corn intrinsic and recombinant
DNA fragments and CrylAb protein in the gastrointestinal contents of pigs
fed genetically modified corn Bt11. J Anim Sci 2003, 81, 2546-51.
- Dutton A, Klein H, Romeis J and Bigler F. "Uptake of Bt-toxin by herbivores
feeding on transgenic maize and consequences for the predator Chrysoperia
carnea", Ecological Entomology 2002, 27, 441-7.
- Romeis J, Dutton A and Bigler F. "Bacillus thuringiensis toxin
(Cry1Ab) has no direct effect on larvae of the green lacewing Chrysoperla
carnea (Stephens) (Neuroptera: Chrysopidae)", Journal of Insect Physiology
2004, in press.
- Dutton A, Romeis J and Bigler F. "Assessing the risks of insect resistant
transgenic plants on entomophagous arthropods: Bt-maize expressing Cry1Ab
as a case study", BioControl 2003, 48, 611"36.
- Ho MW. FAQs on genetic engineering. ISIS tutorial www.i-sis.org.uk
- Ho MW. Genetic Engineering Dream or Nightmare? TWN, Gateway, Gill &
Macmillan, Continuum, 1998, 2nd ed. 1999, re-issued 2003 in cd
- Ho MW. The best kept secret of GM crops. Science in Society 2002,
- Ho MW. GM maize approve on bad science in the UK. Science in Society 2002,
- Collonier C, Berthier G, Boyer F, Duplan M-N, Fernandez S, Kebdani N,
Kobilinsky A, Romanuk M, Bertheau Y. Characterization of commercial GMO
inserts: a source of useful material to study genome fluidity. Poster presented
at ICPMB: International Congress for Plant Molecular Biology (n°VII),
Barcelona, 23-28th June 2003. Poster courtesy of Dr. Gilles-Eric Seralini,
Président du Conseil Scientifique du CRII-GEN, www.crii-gen.org;
also "Transgenic lines proven unstable" by Mae-Wan Ho, ISIS Report, 23 October
- Ho MW. Living with the Fluid Genome. TWN & ISIS, 2003.
- Ho MW. Unstable transgenic lines illegal. ISIS Report 3 December 2003;
also Science in Society 2004,
- Ho MW and Cummins J. Comment on Assessment Report C, submitted to UK
ACRE and European Food Safety Authority 6 April 2004 on behalf of ISIS and
- Ho MW and Burcher S. Cows ate GM maize and died. Science in Society 2004,
- El Estado espanol retirara un OGM a instancias de la UE. El maiz Bt 176
podria provoca resistencias a los antibioticos, GARA, Spain
- Ho MW. Syngenta's Spanish Trojan horse. Science in Society 2004,
- Pusztai A, Bardocz S and Ewen SWB. Genetically modified foods: Potential
human health effects. In Food Safety: Contaminants and Toxins, (J
P F D'Mello ed.),
Scottish Agricultural College, Edinburgh, CAB International, 2003.
- Fares NH and El-Sayed AK. Fine structural changes in the ileum of mice
fed on dendotoxin-treated potatotes and transgenic potatoes. Natural
Toxins, 1998, 6, 219-33; also "Bt is toxic" by Joe Cummins and Mae-Wan
Ho, ISIS News 7/8, February 2001, ISSN: 1474-1547 (print), ISSN:
1474-1814 (online) www.i-sis.org.uk
- Novotny E. Animals avoid GM food, for good reasons. Science in Society 2004,
- Ho MW. Mice prefer non-GM. Science in Society
2002, 13/14, 24.
- Ho MW, Ryan A and Cummins J. Cauliflower mosaic viral promoter - a recipe
for Disaster? Microbial Ecology in Health and Disease 1999 11, 194-7.
- Cummins J, Ho MW and Ryan A. Hazardous CaMV promoter? Nature Biotechnology
2000, 18, 363.
- Ho MW, Ryan A and Cummins J. Hazards of transgenic plants with the cauliflower
mosaic viral promoter. Microbial Ecology in Health and Disease 2000,
- Ho MW, Ryan A and Cummins J. CaMV35S promoter fragmentation hotspot confirmed
and it is active in animals. Microbial Ecology in Health and Disease
2000, 12, 189.
- Ho MW, Lim LC et al. The Case for a GM-Free Sustainable World.
Independent Science Panel Report, ISIS & TWN, 2003 www.indsp.org