Major pollinators apart from the honeybee are suffering steep decline worldwide, chief among them the bumblebee, and neonicotinoid pesticides are a major culprit that should be banned. Prof. Joe Cummins
There has been a huge amount written about the decline of the honeybee, Apis mellifera , but relatively scant attention has been paid to other important pollinators that have also been affected simultaneously, in particular, the bumblebee, Bombus terrestris, which has been experiencing a precipitous global decline. The bumble bee, an important pollinator, has received much less attention than the honeybee because the bumblebee does not produce marketable quantities of honey.
As early as six years ago, there was clear evidence that the bumblebee was in deep trouble in North America. A survey of bumblebee species reached the following conclusion : “The bumblebee subgenus Bombus is represented by five species in North America. Of these, one, B. franklini, may be extinct, and two others, the western B. occidentalis and the eastern B. affinis, appear to be in steep decline. For all of these species, habitat loss and degradation and extensive pesticide use are threats faced daily. However, circumstantial evidence indicates that the principal cause for these population declines is the introduction of exotic disease organisms and pathogens via trafficking in commercial bumblebee queens and colonies for greenhouse pollination of tomatoes.”
In 2009 the decline in Midwestern North American bumblebee was documented and large scale agricultural intensification implicated as the cause . A 2010 report showed that the relative abundances of four species have declined by up to 96 percent and that their geographic ranges contracted by 23–87 percent within the last 20 years . It also showed that declining populations have significantly higher infection levels of the microsporidian pathogen Nosema bombi and lower genetic diversity compared with co-occurring populations of the stable (non-declining) species. It concluded : “Higher pathogen prevalence and reduced genetic diversity are, thus, realistic predictors of these alarming patterns of decline in North America, although cause and effect remain uncertain.”
A number of causes have been put forward for the decline of the bumble bee, these include intensification of land use, introduction of pathogen-bearing bees, pathogens from honey bees, inbreeding, and exposure to pesticides. Before discussing those causes, I describe the lifestyle of the bumblebee.
The bumblebee may look like a large economy-sized honeybee, but it has a distinctly different lifestyle . Most species live in small colonies, usually underground, often in an old mouse hole. The queen lays her eggs in a hollow nest of moss or grass at the beginning of the season. The larvae are fed on pollen and honey, and develop into workers. All the bees die at the end of the season except fertilized females, which hibernate and produce fresh colonies in the spring. Bumblebees form colonies. These colonies are usually much less extensive than those of honeybees. This is due to a number of factors including: the small physical size of the nest cavity, a single female is responsible for the initial construction and reproduction that happens within the nest; and the restriction of the colony to a single season (in most species). Often, mature bumblebee nests will hold fewer than 50 individuals. Bumblebee nests may be found within tunnels in the ground made by other animals, or in tussock grass. Bumblebees sometimes construct a wax canopy (“involucrum”) over the top of their nest for protection and insulation. Bumblebees do not often preserve their nests through the winter, though some tropical species live in their nests for several years (and their colonies can grow quite large, depending on the size of the nest cavity). In temperate species, the last generation of summer includes a number of queens that overwinter separately in protected spots.
The queens can live up to one year, possibly longer in tropical species. Bumblebee nests are first constructed by over-wintered queens in the spring (in temperate areas). Upon emerging from hibernation, the queen collects pollen and nectar from flowers and searches for a suitable nest site. The characteristics of the nest site vary among bumblebee species, with some species preferring to nest in underground holes and others in tussock grass or directly on the ground. Once the queen has found a site, she prepares wax pots to store food, and wax cells into which eggs are laid. These eggs then hatch into larvae, which cause the wax cells to expand isometrically into a clump of brood cells.
After the first or second group of workers emerge, they take over the task of foraging and the queen spends most of her time laying eggs and caring for larvae. The colony grows progressively larger and at some point will begin to produce males and new queens. The point at which this occurs varies among species and is heavily dependent on resource availability and other environmental factors. Unlike the workers of more advanced social insects, bumblebee workers are not reproductively sterile and are able to lay haploid eggs (with one set of chromosomes) that develop into viable male bumble bees. Only fertilized queens can lay diploid eggs (with two sets of chromosomes) that mature into workers and new queens. New queens and males leave the colony after maturation. Males in particular are forcibly driven out by the workers. Away from the colony, the new queens and males live off nectar and pollen and spend the night on flowers or in holes. The queens are eventually mated, often more than once, and search for suitable location for diapause (dormancy).
Bumblebees generally visit flowers that form recognizable groups according to pollinator type. They can visit patches of flowers up to 1–2 kilometers from their colony. Bumblebees will also tend to visit the same patches of flowers every day, as long as nectar and pollen continue to be available, a habit known as pollinator or flower constancy. While foraging, bumblebees can reach ground speeds of up to 15 metres per second (54 km/h). Once they have collected nectar and pollen, they return to the nest and deposit the harvested nectar and pollen into brood cells, or into wax cells for storage. Unlike honey bees, bumblebees only store a few days’ worth of food and so are much more vulnerable to food shortage.
The decline of bumblebee worldwide was brought to light when the impact of particular land use practices was deemed to require fuller study to pinpoint those practices that would benefit the survival of the bumblebee . Organic farming practices increased bumblebee species richness over conventional farming practices . Agricultural landscapes with organic crops support bumblebee density by 150 percent over conventional farming . Pollinator species such as the bumble bee that nest below ground were found to be more adversely effected by tillage than pollinators that nest above ground . It was clear that organic farming practices generally support survival of the bumble bee.
The levels of insecticides that kill bees outright may be much higher than the levels interfering with foraging ability of the bees. Neonicotinoid insecticides are used throughout the world; there is clear laboratory evidence that the neonicotinoid insecticides imidacloprid, thiamethoxam and thiacloprid interfered with reproduction and foraging of the bumblebee . Insecticides used to control pests on canola crops were found toxic to the bumblebee, these include imidacloprid, clothianidin, deltamethryn, spinosad and novaluron . Lower bumblebee species richness was found in the more intensively farmed basin with higher pesticide loads . The naturally derived insecticide spinosad was also found to interfere with bumble bee foraging when applied at sub-lethal doses . Bacillus thuringiensis aizawai (Xentari) proved lethal to the bumblebee when applied in water. In contrast, Bt kurstaki (Dipel) was not toxic to the bumblebee. It seems clear that agricultural insecticides are a threat to the bumblebee , and they should be regulated to prevent bee decline.
There is now good evidence that sublethal levels of pesticides weaken the immune system of the honeybee, leaving them much more susceptible to disease agents such as the parasites Nosema  (see also  Ban Neonicotinoid Pesticides to Save the Honeybee, SiS 49). It is likely that the bumble bee exposed to sub lethal levels of neonicotinoid insecticides may also be more sensitive to infection, as well as suffer from behavioural impairment, but the relevant research has not been done. Parasites and pathogens can spread rapidly between colonies and also between the honeybee and the bumblebee.
RNA viruses contribute to colony collapse, and also expand the viral host range, which could lead to a deeper impact on the health of the ecosystem. Analyses showed that these viruses are disseminating freely among the pollinators via the flower pollen itself. Notably, in cases where honeybee apiaries affected by CCD harboured honeybees with Israeli Acute Paralysis virus (IAPV), nearby non-Apis hymenopteran pollinators also had IAPV, while those near apiaries without IAPV did not. In containment greenhouse experiments, IAPV moved from infected honeybees to bumblebees and from infected bumblebees to honeybees within a week, demonstrating that the viruses could be transmitted from one species to another. This study adds to our present understanding of virus epidemiology and may help explain bee disease patterns and pollinator population decline in general .
The deformed wing virus of honeybee was observed in bumblebees where they pose as serious threat . Honeybee workers can be recruited (by bumble bees) for the establishment of bumblebee nests. Nest establishment rates in three western bumble bee species can be increased dramatically by the addition of either honey bee workers or workers from a second related bumble bee species at colony initiation . The co-mingling of honey and bumble bees in nest establishment shows how viruses may be spread by direct contact between bee genera as distantly related as , for example , pigs and people.
Foraging bees discriminate rewarding from non-rewarding flowers on the basis of colour and odour. Natural and experimental infection by a protozoan parasite (Crithidia bombi, which lives exclusively within the gut tract), impaired the ability of foragers to learn the colour of rewarding flowers. Parasitic infection can thus disrupt central nervous system pathways that mediate cognitive processes in bumblebees and as a consequence, can reduce their ability to monitor floral resources and make economic foraging decisions. This infection-induced change to cognitive function in bumblebees suggests communication between the immune and nervous systems .
Automated video-tracking was used to quantify networks of physical contact among individuals within colonies of the social bumblebee Bombus impatiens. Network structure was found to determine pathogen transmission in naturally and artificially infected hives with Crithidia bombi in social insect colonies . The fungal pathogen Nosema bombi was found to be transmitted horizontally among bumblebee adults, but the transmission was relatively low for the strains of pathogen studied . Commercially-reared bumblebees, used extensively to pollinate greenhouse crops may allow escape of bees from the greenhouse (spill over). Spill over has allowed the gut parasite Crithidia bombi to invade several wild bumblebee species near greenhouses. Given the available evidence, it is likely that pathogen spill over from commercial bees is contributing to the decline of wild Bombus in North America .
The small relatively isolated colonies of the bumblebee are subject to inbreeding as the population declines. When the queen mates with her brothers, the offspring may be subject to inbreeding depression (decrease in fitness and vigour). Such impact is observed in both animals and plants. One study showed that inbred offspring of the bumblebee were smaller but not highly unfit ; though another study in the same species did not detect inbreeding depression in size or immune response .
One mechanism whereby genetically impoverished populations may become extinct is through decreased immunocompetence and higher susceptibility to parasites. The impact of parasitism will increase, pushing threatened populations closer to extinction . Bumblebees have a novel immune system control called density-dependent prophylaxis. Adult bumblebee workers exhibit rapid plasticity in their immune function in response to social context. Density-dependent prophylaxis does not depend upon larval conditions, and is likely to be a widespread and labile response to rapidly changing conditions in adult insect populations. Immune function increases in bumblebee workers as the colony ages (and increases in density). This has obvious ramifications for experimental analysis of immune function in insects, and serious implications for our understanding of the epidemiology and impact of pathogens and parasites in spatially structured adult insect populations .
The decline of the bumblebee was discussed in 2008 and remedies were put forward. Suggested measures include tight regulation of commercial bumblebee use and targeted use of environmentally comparable schemes to enhance floristic diversity in agricultural landscapes . But these are not enough.
Agricultural pesticides known to affect the behaviour and resistance to pathogens of the bumblebee should be banned. The viruses and parasites known to infect bumble bee must be identified and their spread should be restricted as much as possible. The immediate key to the conservation of the bumble bee is the elimination of pesticides that impair their behaviour and resistance to pathogens from the bees environment. Organic farms and wild areas should be set aside as refuges for the bees. Urban bans on cosmetic pesticide use will provide vital refuges for bumblebees and honeybees as well. Currently, homeowners are deluged with advice for eliminating bumblebees and their habitats from urban property. The endangered bumblebee should be protected from intentional Bombuscide by law. Bumblebees and honeybees are inseparable inhabitants of our ecosystem, and both must be preserved.
Article first published 11/02/11
Got something to say about this page? Comment
There are 6 comments on this article so far. Add your comment above.
Gene Sperling Comment left 11th February 2011 18:06:23
Thank you for the amazing article on the Plight of the Bumblebee. It is enlightening see new 'links' indentified that add more and more scientific validity to the destructiveness of these 'cides' on our ecosystems. Anecdotally, I am observing the same phenomena in humans who store or are exposed to pesticides, herbicides, cleaning solvents and artificial scents within the home. These same toxins appear to be increasing the sensitivity of humans thereby leading to a growing number of immune system symptoms. One observation is that there seems to be a direct correlation (in California) between the explosion of newly developed surfactants over the past 5-10 years. These purposeful chemicals increase the absorptive capacity of plants and animals by factors of 10’s and 100’s. These surfactants sole purpose is to deliver an increase in the ‘effects’ of toxins directly to the target and to allow their actions to linger. In humans, it seems that this mechanism is intensifying the response of the upper respiratory tract to invasive mycotoxins, fungi, bacteria and viruses. These pathogens were previously handled by an effective immune system, and now they are allowed absorption in levels that overcome the body’s defense mechanisms. The United States has been particularly at fault as a result of the ‘dismantling’ of the very agencies that would oversee the chemical industry. Maybe the Bumblebee will save us. Cheers, Gene
Rory Short Comment left 12th February 2011 10:10:29
Delight at the information on bumble bees in this article, deep, deep, heart-wrenching sadness at the relentless destruction of our fellow creatures, and consequently ourselves, by fellow human beings in the pursuit of monetary gain.
Mark Peer Comment left 13th February 2011 08:08:50
According to your report Bacillus thuringiensis aizawai is deadly to the bumble bee. Are you aware that Bacillus thuringiensis aizawai has been inserted into the DNA of two types of GE maize? TC1507 Mycogen; Pioneer (Dupont) cry1Fa2 from Bacillus thuringiensis var. aizawai Canada 2002 pat (phosphinothricin N-acetyltransferase) from Streptomyces viridochromogenes United States 2001 DAS-06275-8 DOW AgroSciences LLC cry1F from Bacillus thuringiensis var. aizawai United States 2004 pat (phosphinothricin N-acetyltransferase) from Streptomyces viridochromogenes The full report is here: http://www.fondazionedirittigenetici.org/rassegna/195_STUDIO%205%20-%20ogm%20e%20micotossine%20studipazzi_en.pdf Could the pollen from this maize affect the plight of the bumblebee? You will see the timing of the bumblebees deaths coincide closely of when the maize would have been in widespread use.
joe cummins Comment left 16th February 2011 15:03:00
In reply to Gene Sperling thank you for pointing out the problem of surfactants in pesticide formulations. The so called inert substances in pesticide formulations have been inadequately tested and the actual chemicals have been given trade secret status. The organic authority comments: current “pesticide regulation in North America requires that all pesticides for sale be labeled with specified information, either by the EPA (United States) or the PMRA (Canada). Pesticides must be labeled with the total percentage of active ingredients, and provide an itemized list of the active ingredients. Pesticide manufacturers and retailers, however, are not required to provide an itemized list of inactive ingredients, but are only required to list them as a bulk percentage. This makes it difficult or impossible to determine what ingredients are actually included in different pesticide formulations.” http://www.organicauthority.com/organic-living/organic-living/inactive-ingredients-in-pesticides-may-not-be-safe.html The FIFRA law governing pesticide use definition of pesticide a “mixture” of substances intended to kill a pest. The ‘inert’ substances in pesticides are not tested and their actual makeup is allowed to be held secret .The FIFRA law governing pesticide use definition of pesticide – i.e., a “mixture” of substances intended to kill a pest seems to include surfactants but the surfactants do not appear to have been tested adequately. Neonicotinoid pesticides often include surfactans such as Organosilicone Surfactant which have been found to impair mammalian sperm. However, such surfactants need not be disclosed on the pesticide labels.
jcummins Comment left 14th February 2011 10:10:59
Replying to Mark Peer who asked “Are you aware thuringiensis aizawai has been inserted into the DNA of two types of GE maize?” Yes I am aware that the gene from toxin from thuringiensis aizawai CRY1F has been approved for use in maize and that gene is used by both Dow Chemical and Monsanto in their stacked variety called Smart Stax. The CRY1F gene is also used in cotton. In the experiment used in my article the bumble bees were treated either with sugar water containing thuringiensis aizawai spores or pollen which had been soaked in a solution containing thuringiensis aizawai spores. The bumble bees treated with the sugar solution containing spores failed in reproduction while the bees fed the soaked maize pollen did not fail in reproduction. Furthermore, the CRY1F produced in GM corn and GM cotton is a synthetic approximation of the bacterial gene. One problem may be the unwillingness of the corporations to provide or allow independent experimentation with their patented corn or cotton. The survival of the bumble bee is at stake so the CRY1F used in GM corn and cotton should be studied by independent investigators. Thanks again Mark Peer for your valuable comment on Bt corn and the CRY1F impact on bumble bees. Perhaps we could try to convince the governments to do those studies needed to protect the bees not just the corporations
Todd Millions Comment left 16th February 2011 08:08:25
All corn stocks must be regarded as being contaminated with unstable unmarkered bt inserts,since 1990's.So testing is problematic,type and dosage would be in 'snow drift' patterns simialar to actual measured bomb fallout levels -rather than the official and reasurring statistical averages.I'm uncertian about cotton.But before we loss sugar beet and cane to further 'accidental'pollen drift releases-could not feed comparisions be done with the cold climate feed syrups between honey bee hives?This baseline may be compared too the bumble bee varieties that can handle green houses without batting themselves too death against the glazing,being fed the same syrups.I do appreiciate the difficulties of separating the further'beautiful synergies' of these toxins,from the pesticide sprays.Even if you can find beekeeping associations that WON'T take grant monies from the boiweenie posioners that are killing their bees and them.