Open access to science eprints
TheSparc provides both scientists and the general public free open access to scientific papers that are important for the survival of people and planet
ISIS Report 13/12/10
Virus Nucleic Acid Contaminate Many Vaccines
Risks of cancer and
creation of new pathogens should not be underplayed by regulators Prof. Joe Cummins
A fully referenced
version of this article is posted on ISIS members website and is available for download here
Please circulate widely and repost, but you must give the URL of the original and preserve all the links back to articles on our website
Garbage viruses and DNA in vaccines
Vaccines are currently produced using fertilized
chicken eggs, cell culture or a combination of egg
and cell culture. An ‘attenuated’ vaccine is created from a pathogen by
reducing its virulence, but still keeping it viable, in contrast to those
produced by ‘killing’ the virus (inactivated vaccine). Inactivation is done by selecting
non-pathogenic strains of the pathogen after treatment such as heat or cold
culture, or targeted deletion of virulence genes.
Many live attenuated vaccines are produced using cell
culture. A number of such vaccines have been found to contain not only the
live attenuated viral pathogen but also contaminating viruses or viral nucleic
acid . These contaminants are garbage, and people administering such
vaccines should inform patients of potential risks associated with the garbage.
Recently, the United States Food and Drug Administration (FDA) acknowledged the
contamination of the live attenuated rotavirus vaccine (to prevent traveller’s
diarrhoea) and suspended the vaccine, but later decided that the benefits of
the vaccination outweigh potential contamination risks . The FDA opinion is
premature because the circovirus contaminating the vaccine is active in
replication, transcription and translation of viral genes and able to produce
toxic products. Contaminated vaccines are not isolated cases, they are
Lessons from SV40
Simian virus 40 (SV40)
is a monkey virus inadvertently administered to human populations in
contaminated vaccines produced in SV40-infected monkey cells. Molecular biology
and epidemiological studies suggest that SV40 may be contagiously transmitted
in humans by horizontal infection, independently of the earlier administration
of SV40-contaminated vaccines. In humans, SV40 has been found associated at
high prevalence with specific tumour types such as brain and bone tumours,
mesotheliomas and lymphomas and with kidney diseases . SV40
was discovered as a contaminant of poliovirus vaccine lots distributed to
millions of individuals in the United States between 1955 and 1963; and contaminated
vaccine batches were later circulated worldwide. After SV40 was observed to
cause animal and human cell transformations in culture, and tumour formations
in animals, researchers began to search for SV40 in human cancers . For
example, a 2005 study undertaken in Costa-Rica showed that SV40 is
significantly associated with cancers of the immune system . US FDA
acknowledges that the SV 40 virus (simian virus 40 from
monkey kidney cells) was in the early polio vaccines and its risks : “The
experience in the early 1960s with SV40 contamination of poliovirus and
adenovirus vaccines and the continuing questions regarding whether SV40 could
be responsible for some human neoplasms [cancers] underscores the importance of
keeping viral vaccines free of adventitious agents. “ (See also Flu
Vaccines and the Risk of Cancer, SiS 44 ).
SV40 contamination of polio vaccines is an old lesson that seems to have been
ignored in the current rush to profit from manufacturing vaccines.
Numerous vaccines for humans are contaminated
There are numerous cases
of documented contaminated vaccines intended for humans . Measles vaccine Attenuvax grown in chicken embryo fibroblast cells
was contaminated with Avian leucosis (myeloid leucosis cancer virus) and avian
endogenous retrovirus. Yellow fever vaccine YFvax grown in chicken embryo fibroblast
cells was contaminated with avian endogenous retrovirus. Herpes 3 vaccine Varivax
grown in MRC-5 human cells from aborted foetuses
was contaminated with human endogenous retrovirus K. Rota virus vaccine Rotarix
grown in Vero E6 (African green monkey ) cells was contaminated with with
porcine circovirus 1 and porcine circovirus 2. Rotavirus
Rotateq vaccine grown in Vero (African monkey) cells had Baboon endogenous
retrovirus as contaminant. Measles mumps vaccine MMR II grown in chicken
fibroblast cells had Avian endogenous retrovirus and human endogenous
retrovirus K as contaminants; and Rubella vaccine grown in WI-38 human
diploid lung fibroblast cells was contaminated with Human endogenous retrovirus
K. Rubella vaccine meruvax II grown in WI-38 human lung fibroblast cells contained
human endogenous retrovirus-K.
Veterinary vaccines are similarly contaminated. The genomes
of all animal species are colonized by endogenous retroviruses (ERVs). Although
most ERVs have accumulated defects that render them incapable of replication,
fully infectious ERVs have been identified in various mammals. A feline
infectious ERV (RD-114w) was isolated from many live attenuated vaccines for
pets. Isolation of RD-114 was done independently in two laboratories using
different detection strategies and from vaccines for both cats and dogs
commercially available in Japan or the UK. The study shows that the methods
currently employed to screen veterinary vaccines for retroviruses are inadequate
and should be re-evaluated . Tests of veterinary vaccines for viral
contamination in Hungary found that a torquetenovirus
(TTV), a very small circular single stranded DNA virus, was present in many
vaccines including avian vaccines. The presence of any extraneous agent may
have a significant impact on the safety of the vaccine .
A rogues’ gallery of
vaccine contaminating viruses and DNA
(myeloid leukosis cancer virus)
Avian leukosis virus (ALV-J) appears to be a recombinant of an exogenous avian leukosis virus (ALV) with an envelope
(env) gene probably originating from an endogenous (subgroup E) ALV. ALV-J can infect cell cultures from other avian species, but not mammalian cells. No
genetically resistant meat-type strain of chickens has been found to date.
Commercial Leghorn chickens appear to be resistant to tumour development, but
they may be susceptible to infection. Most tumours associated with ALV-J infection are expressed as myeloblastomas or myelocytomas . Even though the bird cancer
virus does not appear to infect mammals, the persistent exposure of young human
may select mutations of the virus that are virulent in people; and virulent
recombinants can always be created with endogenous human viruses.
Avian endogenous retrovirus
Avian endogenous retrovirus (AER) are a highly diverse group comprising many inserts into the
chicken genome. There are three families of such endogenous retroviruses, related
respectively to avian sarcoma or leukosis cancer virus, mouse leukemia viruses,
and human endogenous retroviruses. Most of the AER are dormant in the chicken
chromosomes, but several are active and capable of making RNA
transcripts . The active transcripts may replicate by reverse transcription
and recombine with related viruses.
Human endogenous retrovirus K
retroviruses (HERVs) are suspects in some autoimmune diseases, in particular,
multiple sclerosis; a member of the family of human endogenous retrovirus W has
been identified as “MS-associated retrovirus” (MSRV). HERVs comprise nearly 8
percent of the human genome, with 98 000 elements and fragments ; all
appear to be defective, containing nonsense mutations or major deletions, and
cannot produce infectious virus particles. Most are remnants of viruses that
integrated many millions of years ago. However, one family – HERV-K (comprising
less than 1 percent of HERV elements) - have been active since the divergence
of humans and chimpanzees, and is one of the most studied. There are
indications it has even been active in the past few hundred thousand years, as
some human individuals carry more copies of the virus. The lack of elements
with a full coding potential within the published human genome sequence
suggests that the family is less likely to be active at present . HERV-K
contaminants in vaccines should not be considered innocuous as they may
recombine with related viruses or with viral sequences in the human chromosome.
retrovirus (BERV) is a inactivated retroviral sequence. BERVs are also found in
the African green monkey . BERV circulating in the bloodstream of humans could
conceivably mutate and recombine to form a virus that could spread rapidly in
the human population because the virus is new to the immune repertoire of the
An infectious endogenous
retrovirus was discovered in live attenuated vaccine for cats and dogs. EVR
RD-114 is related to other oncogenic virus such as feline leuekemia virus and
mouse leukemia virus 83 .
circovirus 1 and porcine circovirus 2
The pig circoviruses
are small circular single stranded DNA viruses. Type 1 virus does not cause
illness in pigs while type 3 virus causes a serious wasting disease of young
pigs. The viruses are frequently found infecting mammalian cell lines. Circovirus
type 1 and type 2 infect many human cell types. Type 1 virus proliferates
without causing distinct cell damage while type 2 virus does . Type 2 virus
causes cytoskeleton rearrangements in dendritic cells, leading to
immunosuppression . Porcine circovirus is lodged in the cell nucleus where
it is replicated. Replication is by a rolling circle mechanism where the single
stranded viral chromosomes are rolled off a double stranded replicative master.
The virus is so small that it only has room for a few genes including two genes
for initiating DNA replication along with genes for nuclear localization and
viral coat protein and a few genes for virulence . The host cell nucleus
provides the enzymes for DNA replication .
(TTVs) are vertebrate infecting, single-stranded circular DNA viruses. Two
genetically distinct TTV groups (TTV1 and TTV2) infect swine worldwide with
high prevalence. Currently, swine TTVs are considered non-pathogenic, although
TTV2 has been linked to post-weaning multisystemic wasting syndrome, a porcine
circovirus disease TTV replicates similarly to the circovirus but is much
smaller than the circovirus . TTV is often presumed to be non-pathogenic, and
is distributed widely among mammals including humans. TTV infection is widely dispersed
in the human population and the virus has been found to accumulate in the
central nervous system and implicated in dementia . Children with recurrent
pneumonia have been found to lack ciliary motility associated with high level
infection of ciliarycells with TTV .
Human and veterinary
vaccines have been found contaminated with wide array of viruses that are
deemed harmless or less risky than the attenuated live virus of the vaccine.
These contaminating garbage viruses are nowhere near as well investigated than
they should have been prior to the commercialization of the vaccines. The
contaminating garbage viruses are deemed harmless because they do not elicit
sera conversion (production of antibody) even though the garbage viruses
frequently produce proteins that are toxic in specific tissues. The
contaminating garbage vaccines are actively cytotoxic in some cases, and
potentially so in other cases by mutation or recombination to create new retroviruses
that are life threatening. Among the garbage viruses, the small circular single
stranded DNA viruses deserve special attention as they are so widespread in the
human and animal populations. Such widespread dispersal of TTV and circoviruses
could cause disaster. The first step in dealing with the garbage viruses is to
provide informed consent to those being vaccinated with contaminated vaccines.
The second is to carry out post-release monitoring for potential hazards from
mutation and recombination, as highlighted in this article.
There are 8 comments on this article so far. Add your comment
|patrons99 Comment left 15th December 2010 21:09:24|
Vaccine-associated diseases are becoming more and more common. What if the vaccine schedules, mandated jabs, and the heavily-vaccinated public are acting as "portals of disease"?
|Karen Comment left 15th December 2010 12:12:02|
Why is Big Pharma allowed to contaminate the human population? Where are our scientists? If they were to organize and not allow the big corps to threaten them maybe we could do away with this barbaric antiquated health system. Stop the lobiests and big greedy corps and people. And take back our right to choose. The Global interests are taking away our rights and people are not paying attention at all. I believe in a global mental connection though, so please people educate yourselves and stand up for your right to choose. We will lose our basic rights to choose organics, supplements, and the choice of healthcare practice. This is a very real threat! Educate yourselves! And PLEASE scientists and doctors who know or suspect the truth, stand up to these horrors of humanity that don't care about people but only about money.
|luis Sabini Comment left 14th December 2010 10:10:30|
What about the footnotes? I couldn't find all the 19...
|joe cummins Comment left 15th December 2010 21:09:47|
In reply to luis Sabini who asks ‘ What about the footnotes? I couldn't find all the 19...’ Thank you Luis Sabini. The reference list is available to ISIS members by clicking on the link fully referenced version on the title page of ISIS Report 13/12.10. I double checked the references and found an error under the heading Feline infectious ERV (RD-114) the reference number should be  not  otherwise the list is complete and accurate.
|Patricia P. Tursi, Ph.D. Comment left 7th January 2011 17:05:45|
According to the book, Dr. Mary's Monkey, the monkey virus was known to infect the polio vaccine prior to the dissemination, but was given anyway, reportedly so no monies were lost. It was not inadvertent. (This book is fascinating because of the reported research involvement of Lee Harvey Oswald. Gary Matsumoto, in his astounding book, Vaccination A, focuses on the debilitating effects of the adjuvants, such as squalene, added to induce an immune response and increase the vaccine effect. According to Matsumoto, the adjuvants can have a more deleterious effect than other vaccine contents, including viruses. This is excellent article and makes me wonder about the focus on Wakefield. A report of the strong correlation between autism and vaccines, came from CDC, and was based on thousands of reports, not an N of 12.
|joe Comment left 24th January 2011 10:10:18|
In reply to wise duck your use of the term "dishonest" for my report which was a simple report on scientific peer reviewed publications is clearly libelous. You claim to be a microbiologist but you do not seem to have read my article or the references I provided. You obfuscated the main point in my report which was the failure of authorities to fully and truthfully disclose the contents of commercial vaccines. My dear duck you are simply a quack !
|Wiseduck Comment left 23rd January 2011 23:11:19|
Wow, I haven't seen many articles this dishonest.
You outright admit several of these viruses can't even infect mammals. Not to mention ERVs aren't viruses. They are "dead."
Even then naked DNA is completely harmless. It cannot enter cells unassisted. Ask anyone who has ever tried to transfect anything.
This is clearly an attempt at fear mongering. Unfortunately, it seems to be working.
|Ron Comment left 10th February 2011 21:09:14|
Very fascinating article. FYI, there is now evidence that HHV-6 can integrate its viral DNA into the host genome of human beings and attach to telomeres, implicated in cancer. Since chickens are afflicted with Marek's disease, antibodies to the latter have been found in humans, and HHV-6 and Marek's are similar and work via similar pathways, is it a stretch to suggest that chicken-embryo based vaccines pose a significant risk to human populations?