A dangerous remedy that may bring malaria back worse than before. Prof. Joe Cummins
We first reviewed the use of transgenic mosquitoes in 2001, when they appeared ready to be released in the field  (Two takes on Malaria -Transgenic Mosquitoes Coming-Rolling Back Malaria*, SiS 13/14). But alas, they have yet to leave the confines of the laboratory, and may not do so until well after malaria has been eradicated. The use of genetically engineered mosquitoes to eradicate the parasite-transmitting mosquitoes have not yet advanced to a state that would allow their safe and effective release to the environment.
The strategy involves introducing transgenes into wild mosquito populations that will render the mosquitoes resistant to infection by the malaria parasite plasmodium. The transgenic mosquitoes must be assimilated into the wild malaria-transmitting mosquito population by a process called gene drive. Gene drive requires introgression of antiparasite genes into the insect vector populations. Such genes, if present at high enough frequencies, will impede transmission of the target parasites and result in reduced human sickness or death .
Transgenic malaria-resistant mosquitoes, modified with a single gene inhibiting parasite growth in the mosquitoes, were found to have a fitness advantage when feeding on parasite-infected blood . This might allow the transgenic mosquitoes to spread in a wild population. The main problem encountered is not so much in finding clever genetic methods to control the parasite in the laboratory as it is to develop gene drive systems that spread the genetically modified malaria mosquitoes safely throughout the entire population of wild malaria mosquitoes. That has been the main hang up.
An example of a potentially viable gene drive system is a gene unit called Medea, discovered in the flour beetle and enhanced synthetically . The synthetic Medea element works by encoding both a maternally expressed toxin and a zygotically expressed antidote. The toxin expressed in the female causes the death of all her progeny that lack the Medea allele, and the antidote rescues Medea-bearing progeny from an otherwise imminent death. In this way, the proportion of Medea-bearing individuals is increased with each generation; and an attached gene conferring resistance to malaria could come along for the ride. Medea is thus able to rapidly spread through a population. Medea is an acronym for maternal-effect dominant embryonic arrest, also alluding to the mythological Greek mother who murdered her children. Medea may produce an effective malaria mosquito gene drive device but that will not be known for some time to come.
Medea is tightly associated with a very large composite transposon Tc1/mariner which is capable of jumping into human genomes. We have previously warned against the release of transgenic insects with transposons that are widely distributed across kingdoms in the living world as a gene drive mechanism  (Stop Release of GM Insects!, ISIS News 9/10). It is tantamount to providing potentially disruptive genome-invaders with wings as well as sharp mouthparts.
A Wolbachia symbiont in Aedes aegypti limits infection with Dengue, Chikungunya (a dengue like virus disease), and Plasmodium Wolbachia are maternally inherited intracellular bacterial symbionts estimated to infect more than 60 percent of all insect species. While Wolbachia is commonly found in many mosquitoes it is absent from the species considered to be of major importance for the transmission of human pathogens. The successful introduction of a life-shortening strain of Wolbachia into the dengue vector Aedes aegypti that halves adult lifespan has been reported . This same Wolbachia infection also directly inhibits the ability of a range of pathogens and parasites to infect this mosquito species. The effect is Wolbachia strain-specific, and relates to Wolbachia priming of the mosquito innate immune system, and potential competition for limiting cellular resources required for pathogen replication. By shortening the mosquito life-span Wolbachia also reduces or prevents the full development of the parasites.The virulent Wolbachia strain wMelPop can survive and replicate when injected into adult female Anopheles gambiae (the most important vectors of malaria in Sub-Saharan Africa), but the somatic infections established are not virulent. These data suggest that stable Wolbachia infections of Anopheles may be possible. The researchers propose using the avirulent Wolbachia modified with transgenes inhibiting parasite development to drive the inhibitor genes through malaria mosquito populations . The term ‘paratransgenesis’ has been coined to describe the genetic manipulation of insect symbiotic microorganisms
Bacteria of the genus Asaia have been proposed as a paratransgenic weapon against malaria. Those symbiotic bacteria are capable of colonizing all of the organs necessary for Anopheles mosquito reproduction. The bacteria are capable of horizontal transfer through an oral route in both adult and larval stages and by venereal route during mating in adults. Asaia bacteria are vertically transmitted from mothers to progeny showing that they may rapidly spread through a population . Paratransgenic Asaia bacteria and other symbiotic bacteria have not yet faced regulatory agencies and the special properties of paratransgenic symbionts require special consideration by the regulators because they may be transmitted again both horizontally and vertically and across taxonomic boundaries.
A mathematical treatment concluded that Medea and Wolbachia bacteria present the best compromise between invasiveness and containment of the gene drives being considered for the control of mosquito born diseases . Another view was put forward by Johns Hopkins University researcher Jason L. Rasgon, who stated : “Replacement of wild-type mosquito populations with genetically modified versions is being explored as a potential strategy to control vector-borne diseases. Due to lower expected relative fitness of transgenic individuals, transgenes must be driven into populations for these scenarios to be successful. Several gene drive mechanisms exist in a theoretical sense but none are currently workable in mosquitoes. Even if strategies were workable, it would be very difficult to recall released transgenes in the event of unforeseen consequences.”
However, commenting on a method called ‘Multi-locus assortment’ that spreads transgenes into vector populations by the release of genetically-modified mosquitoes carrying multiple stable transgene inserts, Rasgon said : “Simulations indicate that insects do not have to carry transgenes at more than 4 loci, transgenes can be maintained at high levels by sequential small releases, the frequency of which depends on the construct fitness cost, and in the case of unforeseen negative non-target effects, transgenes can be eliminated from the population by halting transgenic releases and/or mass releases of wild-type insects.” The remedy for unforeseen negative non-target effects of the transgenes is that the transgenic mosquitoes can be eliminated from the population through the mass releases of wild type mosquitoes! A remedy that unfortunately for the human residents, may bring malaria back worse than ever before. This perhaps summarises the usefulness of the entire approach; even before serious safety concerns have been taken on board.
Article first published 09/06/10
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joe cummins Comment left 16th June 2010 20:08:11
Thank you Todd Millions for your interesting suggestions. Canada's prairie cities such as Winnipeg, Saskatoon and Calgary have annual problems with mosquito born diseases including equine encephalitis which kills and cripples horses and people and West Nike virus. which kill or cripples birds and people. There is no malaria on the prairies as the climate is too cold for the mosquito malaria parasites. The cities of the prairies employ mosquito larvae treatments with Bacillus thuringiensis israelensis or Bti or Methoprene a hormone like insecticide. Larval control is only partly effective in cities such as Winnipeg because clouds of adult mosquitoes are born on the wind from surrounding swamps and muskeg . Tons of adult killing pesticide such as malathion are sprayed over the cities each summer. Tod Millions question about preparing bacterial larvae treatments using compost from Bt containing crops is an interesting one. Bti contains toxin genes that target insects of the family Culicidae (mosquitoes, gnats , midges etc. ) but do not target the moths and bugs controlled by other Bt strains. I do not think that the Bti would be bothered by the crop Bts but the experiment should be done! Another suggestion was to use a coconut mash in water as a mosquito repellent.. That is an interesting suggestion and one worth following up. I note that mosquito coils US Patent 4144318 from 1979 contains coconut flour.Mosquito coil is mosquito-repelling incense, usually shaped into a spiral, and typically made from a dried paste of pyrethrum powder with sawdust and/coconut flour. Mosquito coils are used extensively in Asia and Canada. I used mosquito coils in tents and campsites in Northern Ontario, Quebec,and Manitoba. where that mosquito seems to be the size of a small bird and a very blood thirsty one at that . The smoke, however, is a trade off between repelling big bloodsuckers and being exposed to excessive carcinogens in the coil incense.
Todd Millions Comment left 18th June 2010 05:05:37
Mr Cummins-To clarify my comment-the scraps of coconut flesh where the food for the bacterial cultures in the tests I described-other (waste sugar and or carb sources could be used I think. I'm well familar with equine encephilitius,years ago we lost an exellent sulfur spring mare to the vaccine-the reaction dropped her with in 3 minites.No other horses we vaccinated had this reaction.The vets opinion was-she already had a mild case or had had one and the vaccine caused an allergetic reaction. I'm also very familar with and have personal experince of idiot cousins with spray tickets and malithion poisoning.This was the over the west nile panic-which I thoughht wouldn't ammount to much after the initial rum through-because that had already being the experince in europe-with the bird flyways from africa.Not what the poison weenies wanted to hear of course and now I'm proven right-they have trouble remembering. If I may suggest-you may be a bit complacent over malaria in canada-we do now have the frost resistant tiger mosquito from japan her in the southern praireis-apparently these beauties can host the parasite. Glad you picked up on my notion of trying runs of bt bacturum cultures fed sugar sols from bt insert crops-it sould be done in a proper lab.A russian one perhaps? The in joke I should have probably explained further is-"In canada swallows eat mosquitos,in winnipeg the mosquitos eat the swallows".
Todd Millions Comment left 15th June 2010 21:09:05
Prof Cummins-Good overveiw of this officially plausable nonsence. I recall tv reports (cbc),of tropical tests involving waste sugar source brewing of packets of bt bacturium-coconut fragments in a barrel of warm water,After one week,small amounts of this brew were added to standing water ponds that were breeding malarial winnipeg swallows-The result was a almost complete wipe out at the laval stage.Cost-peanuts compared to incecticidal spray(I'm not sure how costs compare to impregnated bed nets).Guess which approach ,after these tests-the IMF and UN forced poor countries too adopt. Still-I'm just perishing too know-Can you brew bt cultures in sugar solutions contaminated with bt gmo crops? I anxiously await such chicken and modified egg testing! In warm climes-I think large colony size bat roosts would be even better bang for buck-where they hunt,pinpionts the source of the problen very quickly.