ISIS Report 31/08/10
'Homeopathic' Signals from DNA
Nobel Laureate who
discovered the HIV presents controversial but well-documented findings that electromagnetic
signals can be detected from highly diluted solutions of DNA. Dr. Mae-Wan
fully referenced and illustrated version of this paper is
posted on ISIS members website and can be downloaded here
Please circulate widely and repost, but you must give the URL of the original and preserve all the links back to articles on our website
“Luc Montagnier, the
French virologist who won a Nobel prize in 2008 for linking HIV with AIDS, last
week made controversial claims that highly dilute solutions of harmful viruses
and bacteria emit low-frequency radio waves, allegedly from watery
nanostructures formed around the pathogens. Similar claims have been made for
homeopathic remedies.” New Scientist 
Latest round of
attack on homeopathy
Homeopathy has been
subject to periodic attacks from the mainstream medical and scientific
community aided and abetted by uninformed journalist in the mainstream press
eager to create a good impression with the scientific establishment.
The latest round was initiated by a damning
report from the UK Parliament Science and Technology Committee released in
February 2010, Evidence Check 2: Homeopathy , which concludes that
the existing scientific literature shows no evidence that that homeopathy is
efficacious beyond the placebo effect, and that “explanations for why homeopathy
would work are scientifically implausible.” Therefore, the National Health
Service should stop funding homeopathy and the Medicines and Healthcare
products Regulatory Agency should not allow homeopathic product labels to make
medical claims without evidence of efficacy.
In July, the British Medical Association
passed a resolution to stop homeopathy being made available on the National
Health Service (NHS), and to have all homeopathic remedies to be placed in a
special area marked ‘Placebos’ in health shops and pharmacies. However, the UK government is not taking action to ban homeopathy from the NHS , which has funded
homeopathy from its inception in 1948. So homeopathy is safe, at least for now.
Lack of plausible
explanation the major hurdle in gaining public acceptance
The most difficult
hurdle in getting general acceptance for homeopathy is without doubt the lack
of an explanation, based on contemporary science, on why it would work. In my
view, that is more important than getting double-blind, placebo-controlled data
on efficacy. Such an explanation is beginning to emerge, and Luc Montagnier’s
research team may have provided some key observations.
The Nobel Laureate has entered the fray, bravely
picking up on work done by his fellow countryman, the recently deceased
immunologist Jacques Benveniste, who became the centre of a major international
controversy in 1988, when Benveniste and his research team published a paper in
the journal Nature describing the apparent homeopathic action of very
high dilutions of anti-IgE antibody on the human blood cells basophils. As
condition for publishing the paper, the then journal editor John Maddox
organised and subjected Benveniste and his team to a farcical and damaging
public trial  that included illusionist and well-known sceptic James Randi
and fraud expert Walter Stewart .
Montagnier’s recent work, summarily
dismissed in the New Scientist  and elsewhere, has been published in
two papers in 2009, and the evidence presented is clear and informative.
property of DNA”
The first paper
reports the capacity of some bacterial DNA sequences to induce electromagnetic
waves at high dilutions in water , and appears to be a “resonance
phenomenon” triggered by the ambient electromagnetic background of very low
frequency waves. Interestingly, genomic DNA of most pathogenic bacteria contain
sequences that are able to generate such signals, suggesting that highly
sensitive detection system might be developed for chronic bacterial infections
in human and animal diseases. The second paper follows up this suggestion,
showing that it is indeed possible to detect the presence of HIV DNA even when
the RNA of the virus has disappeared from the blood of people infected with HIV
and undergoing antiviral therapy (see  Electromagnetic
Signals from HIV, Prospects for a Science of Homeopathy, SiS 48).
Montagnier and his colleague Claude
Lavallee initially observed that filtering a culture supernatant of human
lymphocytes infected with the bacterium Mycoplasma pirum (about 300 nm
in diameter) through filters with pore size of 100 nm or 20 nm gave apparently
sterile fluid. However, the sterile fluid was able to regenerate the original
mycoplasma when incubated with a mycoplasma-negative culture of human
lymphocytes within 2 to 3 weeks. Similarly, filtering an infective fraction of
HIV particles (120 nm) through 20 nm filter failed to retain the infective
Furthermore, the infectious filtrate
produced electromagnetic waves of low frequency in a reproducible manner after
appropriate dilutions in water. They suspected a “resonance phenomenon”
depending on excitation by the ambient electromagnetic fields such as the 50/60
Hz signals from the mains. The infectious signal appeared associated with
“polymeric nanostructures of defined size” present in the diluted filtrate. The
supernatant of uninfected eukaryotic cells used as controls did not have those
associated with nanostructures
Given the initial
clues, the researcher team set out to investigate the phenomenon more
thoroughly, to characterize the electromagnetic (EM) signals and the
nanostructures produced by the purified bacteria.
In addition to M. pirum, they looked
at E. coli. The supernatants of deliberately infected human lymphocytes
containing 106 or 107 infectious units per ml were
filtered twice first through 450 nm Millipore filters to remove debris, and
then 100 or 20 nm filters to remove mycoplasma cells. The filtrates were
confirmed sterile by incubation for several weeks in enriched growth medium. Repeated
search for traces of mycoplasma DNA by polymerase chain reactions (PCR) was
also consistently negative.
However, when the filtrates were incubated
for two weeks or three weeks with a culture of human activated T lymphocytes,
the mycoplasma was recovered in the medium with all its original
The filtrates were analysed just after
filtration for production of EM waves of low frequency. For this purpose, a
devise previously designed by Benveniste and Coll was used for the detection of
signals produced (see Figure 1).
Figure 1 Detecting EM signals with
Benveniste and Coll’s device
The filtrates were serially diluted 1 in
10, after each dilution, the tube is tightly stopped and strongly agitated on a
Vortex apparatus for 15 seconds. This step, which is equivalent to homeopathic
‘succussion’, has been found critical for the generation of signals.
After all dilutions have been made (15 to
20), the stopped tubes were read one by one on an electromagnetic coil (copper
wire on a bobbin, impedance 300 Ohms), connected to a Sound Blaster Card, itself
connected to a laptop computer powered by its 12 volt battery. Each emission is
recorded twice for 6 seconds, amplified 500 times and processed with different
softwares to visualise the signals on the computer screen. The main harmonics
of the complex signals were analysed by softwares for Fourier transformations.
In each experiment, the internal noise generated by the different pieces of the
reading system was first recorded (coil alone, coil with a tube filled with
ordinary water). Fourier analysis shows that the noise was predominantly very
low frequencies probably generated at least in part by the 50/60 Hz ambient
electric current. Using the 12 volt battery to power the computer reduced the
noise, but did not abolish it altogether; as the noise was found to be
necessary for the induction of the resonance signals from the specific
EM signals did
not decrease with dilution
When the EM signals
from serial dilutions of the M. pirum filtrate were recorded, the first
obvious change was an increase in the overall amplitude of the signals
at certain dilutions over the background noise, and also higher frequencies.
This change was abolished if the tube analysed was placed inside a box shielded
with sheets of copper and mumetal, which also shields static magnetic field as
well as low frequency EM fields.
Fourier analysis of the M. pirum
signals confirmed a shift towards higher frequencies close to 1 000 Hz and
multiples thereof. The profiles were identical and highly reproducible for all
the dilutions showing an increase in amplitude.
The first low dilutions were usually
negative, showing the background noise only, positive signals were typically
obtained at dilutions ranging from 10-5 to 10-8 up to 10-12,
at which the signal was greatest before it became negative at 10-13.
The positive dilutions varied according to
the type of filtration; the 20 nm filtrate being generally positive at
dilutions higher than those of the 100 nm. The original unfiltered suspension
was negative at all dilutions, a phenomenon observed for all the microorganisms
The 20 nm filtrate was centrifuged through
a density gradient to separate components with different densities that were
tested for electromagnetic emissions. The emitting structures were distributed
in a large range of densities from 1.15 to 1.25 gm per ml.
In the experiment with E. coli, supernatants
of cultures containing 109 units/ml were used. No signal appeared
after filtration through 20nm filters, suggesting that the structures
associated with the signals were retained by those filtered, and therefore had
a size greater than 20 nm and lower than 100 nm.
The final filtrate was sterile. Signal
producing dilutions again range form 10-8 to 10-11, with
profiles on Fourier transformation similar to, yet distinct from those of M.
Pirum. In one experiment, some very high dilutions were found to be positive,
ranging from 10-9 to 10-18.
In contrast, the unfiltered supernatant did
not show any signal above background up to 10-38 dilution. This suggests
that the low dilutions are self-inhibitory, probably by interference of the
multiple sources emitting in the same wave length, slightly out of phase, like
radio jamming. Alternatively, the abundance of nanostructures can form a gel in
water and therefore inhibited from vibrating (more later).
EM signal can be
wondered whether or not it was possible to generate new signal-emitting
structures from tube to tube by wave-transfer. The answer was yes.
A donor tube of a low “silent” dilution of E.
coli (10-3) was placed side by side close to a receiver tube of
the positive “loud” highest dilution of the same preparation (10-9).
Both tubes were placed together in a mumetal box for 24 hours at room
temperature, so the tubes were not exposed to external electromagnetic noise
and only exposed to the signals generated by the structures present in the
tubes themselves. When tested after that, the donor tube was still silent, and
the receiver tube too, became silent.
But when further dilutions were made from
the receiver tube, they became positive again. These results suggest that the
receiver tube was made silent by the formation of an excess of new
nanostructures, which could emit signals again upon further dilution. The
effect was suppressed by putting a sheet of mumetal between the two tubes
during the 24 h contact period, pointing to a role of low frequency waves in
EM signals from
all pathogenic bacteria
Emission of similar
EM signals was found with other bacteria such as Streptococcus B, Staphylococcus
aueus, Pseudomonas aerogniosa, Proteus mirabilis, Bacillus
subtilis, Salmonella, Clostridium perfringens, all in the
same range of dilutions as for E. coli, and only after filtrating at 100
nm, not 20 nm. Importantly, the transfer effect between the two tubes, one
silent, one loud, was only observed if both contained dilutions of the same
bacterial species. These results indicate that the signal transfer is
Does the signal depend on the initial
number of cells? To investigate that, a stationary culture of E. coli
was counted and adjusted to 109 cells/ml and serially dilution 1 in
100 down to 1 cell/ml. Each dilution was filtered through 100 nm, then analyzed
for signal emission. Surprisingly, the range of positive dilutions were not
strictly dependent on the initial concentration of E. coli cells, being
roughly the same from 109 down to 10 cells, suggesting that the same
final number of nanostructures was reached at all concentrations.
Was the effect dependent on the operator?
No. Two operators measuring independently the same dilutions of E. coli
produced exactly the same results. The results were also independent of the
order in which the samples were read, whether in descending dilutions from the
lowest to the highest or vice versa. And even in random order. That was
achieved by letting another lab worker place the diluted samples in random
order, the labels being unknown to the person reading the samples. Again the
same results were obtained, provided each tube was well separated from the
others to avoid cross-talk between them. Finally, the results were independent
of the reading site. They were the same in France (Paris), Canada (Montreal) and Cameroun (Yaoundé), even though the background noise at each place was
distinct. The positive signal is always clearly differentiated by the same
higher frequency peaks.
A non-exhaustive survey of the bacterial
species displaying EM signals suggests that most of the bacteria pathogenic for
humans are in this category. In contrast, probiotic non-pathogenic bacteria
such as Lactobacillus and their DNA are negative for EM signal emission.
What is the
nature of the EM signal emitting nanostructures?
were not destroyed by treatments with enzymes that destroy RNA, DNA or protein
(RNAse A, DNAse 1, proteinase K); only by heating at 70 ˚C for 30 minutes,
or freezing for 1 hour at -20 ˚C or -60 ˚C. Treatment with lithium
cations, known to affect H-bonding of water molecules, reduced the intensity of
the signals, while the range of the positive dilution remained unchanged.
traced to specific pathogenic DNA sequences
experiments, the researchers found that treating a suspension of E. coli
with formaldehyde, which killed the bacteria, did not alter the capacity to
induce the EM signals. This treatment denatured the surface proteins of the
bacteria but did not change their genetic material - the double-helical DNA -
and suggests that the source of the signals may be the DNA itself.
Indeed, DNA extracted from the bacterial
suspension by the usual method, after filtering and appropriate dilutions in
water, was able to emit EM signals similar to those produced by intact bacteria
under the same conditions. DNAse treatment of the extracted DNA solution
abolishes its capacity to emit signals, so long as the nanostructures
previously induced by the DNA are destroyed.
The same as for the intact microorganisms, the
isolated DNA must be filtered before the EM signals can be detected in the
diluted solutions. This suggested to the researchers that filtering is
necessary to break up a “network of nanostructures organized in a gel at high
concentrations in water,” allowing them to be dispersed in further dilutions. One
complication is that the filtration through 100 nm pore size filter did not
retain the DNA. The dilutions positive the EMS were in the same range as those
for the intact bacteria, generally between 10-7 to 10-13.
At the high dilution of 10-13,
calculations indicate that there is no DNA molecule larger than 105 Da
in the solution; making it unlikely that the EM signals are produced directly
by the DNA itself, but rather by the “self-sustained nanostructures induced by
the DNA.” Generally, all the bacterial species shown to be positive for EM
signals yielded also DNA preparations positive for EM signals, and they were
In the case of E. coli, some non-pathogenic
strains used for gene cloning were negative. This suggests that only some
sequences of DNA are the source of the EMS.
The signal is linked to the ability of the
bacteria to cause diseases, which in turn depends on the capacity of the
microorganism to bind to eukaryotic cells. They looked in M. prium DNA,
where a single gene – adhesion coding for a 126 kDa protein – is responsible
for the adhesion of the mycoplasma to human cells. The gene was cloned
previously in Montagnier’s laboratory, and they had it as two fragments: 1.5
kbp N terminal part and 5 kbp C terminal part of the protein in two different
plasmids. The two plasmids containing the fragments were amplified in the E.
coli strain that did not produce EM signals.
But when the E.coli strain (XL1blue)
was transformed with either plasmids carrying an adhesion gene fragment, EM
signals were produced.
The two adhesion DNA fragments were then
cut out by specific restriction enzymes and isolated by agarose
electrophoresis. Each DNA fragment was able to induce the EM signal. To confirm
the result, they purified a large fraction of the adhesion DNA from the whole
mycoplasma genome using specific primers and amplication by PCR, and found that
this fragment induced EM signals.
The researchers have discovered a novel
property of DNA, the capacity of some sequences to emit electromagnetic waves
in resonance after excitation by the ambient electromagnetic background. They
speculated that all DNA may be capable of emitting EM signals, but “in our
conditions of detection, it seems to be associated with only certain bacterial
They detected similar EM signals in the plasma
and in DNA extracted from the plasma of patients suffering from Alzheimer,
Parkinson disease, multiple sclerosis, and rheumatoid arthritis, suggesting
that bacterial infections are present in those diseases. They require 20 nM
filtrations suggesting that the nanostructures produced are smaller than those
produced b y bacterial DNA.
Moreover, EM signals can be detected also
from RNA viruses, such as HIV, influenza virus A, Hepatitis C virus, In
patients infected with HIV, EM signals can be detected mostly in patients
treated by antiretroviral therapy and having a very low viral load in their
plasma. Such nanostructures persisting in the plasma may contribute to the
viral reservoir which escapes the antiviral treatment, assuming that they carry
genetic information of the virus.
It is known from the very early X-ray
diffraction studies of DNA that water molecules are tightly associated with the
double helix, and DNA in water solution forms gels associating a large number
of water molecules.
The capacity of diluted solutions to emit
EMS after they have been isolated in mumetal boxes last up to 48 hours,
indicating the relative stability of the nanostructures.
What exactly are these nanostructures and
why do they emit electromagnetic waves? Mantagnier and his team are not very
explicit on this. But we shall examine this more carefully at the end of the
next article in this series .
There are 7 comments on this article so far. Add your comment
|patrons99 Comment left 5th September 2010 22:10:31|
This is a fantastic discovery. Dr Montagnier may well end up with a second Nobel prize for this work. Ultimately, this discovery could lead to in vivo, noninvasive imaging, quantitation, and therapy of MANY diseases which have plagued mankind. The physics of these energy transfers needs to be fully described and understood. The structures of these EMS-emitters needs to be described. Where is the energy coming from? light waves? Perhaps EM signals could be used to detect, quantitate, and localize DNA hypomethylation, and human epigenetics in vivo. Can EM signals be used to distinguish in vivo between endogenous, e.g. HERVs, and exogenous viruses?
|Rory Short Comment left 1st September 2010 10:10:43|
As a Multiple Sclerosis sufferer it was very interesting indeed to read that there could be an infectious agent associated with the disease. I was diagnosed with MS in early 1992 and up until now I have dismissed any idea that there was any infectious agent involved with MS, accepting it as a purely auto-immune disease.
|Todd Millions Comment left 1st September 2010 10:10:57|
Facinating-Like reading Dr.R.Becker again.
The lithium ion attenuation of the generated signal-
Have other metallic ions being compared?Silver for instance,or carbon?
How hard to isolate the 50/60Hz powerline background and try the resonate frequency of the earth(7.5Hz?)?
Maybe 200Hz for a comparision to the powerline background.
I've always regarded Paracelsus as the founder of homopathy therapy-the inverse of his-'dose makes the poison'dictum.He did use this for his immune stimulating therapies.
|tony villar Comment left 1st September 2010 10:10:50|
long live Dr. Hahnemann.
|David Llewellyn Foster Comment left 2nd September 2010 13:01:42|
Dear Dr Ho, as always, your observations are extremely interesting, timely and relevant. With respect to the comment above from Rory Short, this opens up the whole issue of the thought-provoking questions raised in the controversial research of Dr Trevor Marshall - he of the "infamous protocol". Any ideas about this and the role of therapeutic fungi and even cannabis (!) in the elimination of pathogenic microbiota? Kind regards from DLF
|jollyd Comment left 28th April 2011 20:08:17|
Although, one finds it interesting that Dr. Montagnier's work on this theory has been used to discredit him as a wako by AIDS militants and others in the media (pockets of big pharma, knowingly or not).
Here is some related material reported in Science Daily :
Water molecules surround the genetic material DNA in a very specific way. Scientists at the Helmholtz-Zentrum Dresden-Rossendorf (HZDR) have discovered that, on the one hand, the texture of this hydration shell depends on the water content and, on the other hand, actually influences the structure of the genetic substance itself. These findings are not only important in understanding the biological function of DNA; they could also be used for the construction of new DNA-based materials.
|Elizabeth O'Donnell Comment left 30th August 2013 21:09:25|
Luc Montagnier has made a fascinating discovery. If DNA is capable of electromagnetic signalling then perhaps external EM signals can interact with a DNA molecule resulting in DNA methylation therefore silencing gene expression. This could be a possible mechanism for external environmental factors to produce epigenetic effects altering protein synthesis,enzyme and brain neurotransmitter production. This raises the possibility that external variables effect brain function through the epigenetic effects of electromagnetic or low frequency radio waves signalling, especially as the same 7Hz frequency is obtained in the brain.