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Applications
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a)Dogfish egg case as viewed under polarised light.
Hold the mouse over the image to see the corresponding orientation
overlay. b)Image map representing the variation in relative
retardation across the above sample. |
To perform quantitative analysis using this technique,
it is necessary to measure the relative intensity of a small area, as it
is rotated relative to the polarisers. We have developed a computerised
system for analysing larger areas, with our industrial partners Prior
(microscopy), Datacell,
and Ross research. A computer controls a motorised stage, and image
aquisition card, allowing the capture and intensity analysis, of a sample
at a number of orientations. The software (Image pro plus) is then able to
compare the sample at each orientation to determine where the maximum and
minimum intensities occur. From this data, the software can then create an
overlay demonstrating molecular orientation (hold the mouse over the image
to see this).
This technique has been applied to a range of different samples - mainly
fixed biological liquid crystalline arrays, but also thin sections of
stone.
The examples shown are of dogfish eggcasing collagen, and granite. To display the molecular orientation overlay, hold the mouse over the images, and it will appear.
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a)Thin section of granite viewed under polarised
light. Hold the mouse over the image to see the corresponding
orientation overlay.
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