Scientists Confirm Failures of Bt-Crops

In the Bt cotton, the level of CrylAc was significantly lower in boll tips where flowers had remained attached, compared with normal boll tips. Boll tips where the flowers remained attached are often the sites at which corn earworms, Helicopverpa zea (Boddie) penetrate Bt cotton bolls. In both Bt maize and Bt cotton, tissues that had low chlorophyll content also had reduced Cry1A proteins.

The US Environment Protection Agency recommends planting a certain percent of crop area with non-Bt varieties to serve as ‘refuge’, in order to ensure that enough susceptible insects are produced to limit the evolution of resistance. An important requirement for the refuge strategy to work effectively is a high level of expression of the toxin, so heterozygous insects (those with one copy of resistance gene) will fail to survive to reproduce. Thus, any reduction from high toxin levels will compromise the refuge strategy and the effectiveness of Cry1A proteins in pest control.

Researchers at the University of Arizona Tucson and the Arizona Cotton Research and Protection Council, Phoenix had found a “surprisingly high” frequency (0.16) of the Cry1Ac resistance gene in field populations of the pink bollworm in Arizona in 1997, which did not appear to increase further as expected in 1998 or 1999 [5]. However, the tests were done with the recombinant Cry1Ac protein produced in the bacterium, Pseudomonas fluroescens, and not from the Bt cotton plant, and could be giving entirely misleading results on the evolution of resistance in the field (“No Bt resistance?” SiS20) [6].

Bt resistance in Australia

A population of the Australian cotton bollworm, Helicoverpa armigera – the most important agricultural pest in Australia as well as China, India and Africa - has developed resistance to Cry1Ac at 275-times the level that would have killed the non-resistant insect [7]. Some 70 percent of the resistant larvae were able to survive on Bt cotton expressing Cry1Ac (Ingard). The resistance is inherited as an autosomal semi-dominant trait (the heterozygote with one copy of the resistance gene is half as resistant as the homozygotes with two copies of the resistance gene).

Bt cotton varieties expressing Cry1Ac (Ingard) have been grown in Australia to control the cotton bollworm since 1996, and a new variety containing both Cry1Ac and Cry2Ab was commercially released in late 2003. Resistance monitoring in Australia and China had suggested that pest susceptibility to Cry1Ac was declining in the field. In 2001, a strain of cotton bollworm was isolated from the survivors in the New South Wales and Queensland monitoring programme that appeared to be resistant to Cry1Ac. The researchers have now confirmed the findings, and attributed the high level of resistance to a 3- to 12-fold over-expression of an enzyme, serine protease, which binds avidly to Cry1Ac toxin, preventing it from acting, and possibly, detoxifying it by breaking it down.

Canadian scientists find yield and economic disadvantage in Bt maize 

Researchers at the Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, carried out a field experiment over three years to compare commercial corn hybrids with their corresponding Bt-hybrids belonging to the Monsanto and Syngenta [8]. They found that some of the Bt hybrids took 2-3 additional days to reach silking and maturity and produced a similar or up to 12 percent lower grain yields, with 3-5 percent higher grain moisture content at maturity in comparisons with their non-Bt counterparts. Higher grain moisture content increases drying cost. Bt hybrid seeds also have a $25-30 premium per ha.

The economic disadvantages are dwarfed in comparison with impacts on biodiversity and human and animal health that have been known for years, however (see below: also“Bt risks negligible” SiS 2002, 13/14).

Bt maize more woody

It has been known for some time that genetic modification is full of pitfalls, among which are many unintended effects. A paper published in 2001 [9] reported that the content of lignin (woody substances) was high by 33 to 97 percent in the Bt maize varieties tested: Bt11, Bt176 and Mon810. Now, researchers at environmental and agricultural institutes in Leipzig, Aachen and Muncheberg, Germany, and the University of Waterloo in Ontario, Canada, have confirmed increases in lignin in two Bt maize lines, Novelis (event MON00810-6, from Monsanto) and Valmont (event SYN-EV176-9, from Syngenta), compared with their respective isogenic varieties, Nobilis and Prelude, all grown under identical conditions [10]. The increases in lignin are more modest, and are restricted to the stems of the plants: Novelis by 28 percent over Nobilis, and Valmont by 18 percent over Prelude.

Increase in lignin content will impact on the digestibility of the plant for livestock, it also decreases the rate at which the plant material break down, affecting nutrient recycling, the soil microbial community, and soil carbon balance.

Intriguingly, an earlier report has also found increased lignin in Monsanto’s Roundup Ready soya, genetically modified to be tolerant to the herbicide Roundup [11], which caused the stem to split open in hot climate and crop losses of up to 40 percent.

These results suggest that genetic modification per se may be increasing lignin content, perhaps as a response to metabolic stress from the high levels of transgene expression driven by aggressive viral promoters.

Impacts on biodiversity and health

Bt toxins are known to harm beneficial/endangered insect species and soil decomposers [12]:

• Pollen from Bt-maize was lethal to the larvae of the monarch butterfly.
• Increased mortality of lacewing larvae fed on artificial diet containing Bt-maize or on corn-borer larvae that had eaten Bt-corn.
• Bt sprays used to reduce caterpillars in forests led to fewer black-throated blue warbler nests.
• A parasite of corn-borers, Macrocentris cingulum, was found to be reduced in Bt-cornfields compared with non-Bt corn fields.
• One preparation of Bt (var. tenebrionis), reported to be specific for Coleoptera, caused significant mortality in domestic bees.
• Soil-dwelling collembola, Folsomia candida, an important decomposer, suffered significant mortality from transgenic maize with Cry1Ab.
• Bt not only remains in the soil with Bt-plant debris, it is actively exuded from the plant roots where it binds to soil particles and persists for 180 days or more, so its effects on soil decomposers and other beneficial arthropods may be extensive.

Bt-toxins are actual and potential allergens for human beings. Field workers exposed to Bt spray experienced allergic skin sensitization and induction of IgE and IgG antibodies to the spray [13]. Recombinant Cry1Ac protoxin was found to be a potent mucosal immunogen, as potent as cholera toxin [14]. A Bt strain that caused severe human necrosis (tissue death) killed mice infected through the nose within 8 hours, from clinical toxic-shock syndrome [15]. Both Bt protein and Bt-potato harmed mice in feeding experiments [16]. All Bt-toxins along with many other transgenic proteins exhibit similarities to known allergens and are hence suspected allergens until proven otherwise (“Are transgenic proteins allergenic?” SiS 25) [17-19].

Recently, much publicity has been given to a report from scientists in Portugal published in the house journal of the American Academy of Allergy, Asthma and Immunology, because it claimed “lack of allergenicity of transgenic maize and soya samples” [20].

A careful reading of the report reveals, however, that the researchers had no evidence that the small number of subjects they tested have ever been exposed to transgenic maize and soya. They wrote:  “Bearing in mind that since 1998 all the GM products under testing were approved for commercialisation in the European Union.., we assumed that consumption of maize and soya food-derived products implied a consumption of GM soya and maize.” (emphasis added). Moreover, the tests performed were limited to skin pricks and IgE antibodies, both known to be limited in reliability [21]. Most of all, there are many allergies that do not involve IgE antibodies [22].

Nevertheless, the researchers stated, “In this study we did not obtain any differential positive results, which allows us to conclude that the transgenic products under testing seem to be safe regarding their allergenic potential.” (emphasis added).