ISIS Report 26/09/05
Scientists Confirm Failures of Bt-Crops
Ineffective against insect pests, harmful to health and biodiversity, yield
drag, pest resistance. Dr.
A fully referenced
version of this paper is posted on ISIS members’ website. Details
Farmers were first
Scientific studies from many countries have now backed up what farmers
have known for years, that Bt crops – genetically engineered with Bt toxin
proteins from the soil bacterium Bacillus
thuringiensis targeted at insect pests - often failed to protect
against pest attacks, and have other problems as well.
Scientists in India,
China and the United States found that the levels of Bt toxin produced by
Bt crops vary substantially in different parts of the plant and in the course
of the growing season, and are often insufficient to kill the targeted pests.
This could lead to greater use of pesticides, and accelerate the evolution
of pest resistance to the Bt toxin. Pest resistance to a Bt toxin has indeed
arisen in the field in Australia.
The Bt toxins are a family
of similar Cry proteins identified by numbers and letters. Each Cry protein
differs somewhat in amino acid sequence and targets specific pests.
Scientists at the Central Institute of Cotton Research studied Bt cotton
hybrids approved for commercial planting in India: Bollgard-MECH-12,
Bollgard-MECH-162, Bollgard-MECH-184, Bollgard-RCH-2, Bollgard-RCH-20, Bollgard-RCH-134,
Bollgard-RCH-138 and Bollgard-RCH-144. All the varieties were created by using
Indian parent-varieties to which the crylAc gene was introduced from the Bt-cotton
variety, Coker 312, ultimately derived from transformation event MON531 (Monsanto).
The researchers found that
the amount of Cry1Ac protein varied across the varieties and between different
plant parts. The leaves had the highest levels; whereas the levels in the
boll-rind, square bud and ovary of flowers were clearly inadequate to fully
protect the fruiting parts producing the cotton bolls. Increasing numbers
of armyworm (Helicopverpa armigera)
larvae survived as toxin levels went below 1.8 mg /g wet weight of the plant parts.
Thus, a critical level of 1.9 mg/g
was needed to kill all the pests.
Regardless of plant varieties, the level of toxin decreased with the age of
the plant, though the decrease was more rapid in some hybrids than in others.
By 110 days, Cry1Ac expression decreased to less than 0.47mg/g in all hybrids.
In a separate study, scientists
at the same institute tested the susceptibility of an insect pest from different
regions in India to Bt toxin . They took samples of larvae
of the spotted bollworm, Earias vitella
from 27 sites in 19 cotton-growing districts of North, Central and South India
during the 2002 and 2003 cropping seasons and tested their susceptibility
to Cry 1Ac toxin protein purified from E. coli strains expressing the recombinant
protein. The LC50
- the concentration killing 50 percent of the larvae – of Cry1Ac ranged from
0.006 to 0.105 mg/ml.
There was a 17.5 fold overall variability in susceptibility among the districts.
The highest variability of 17.5 fold was recorded from districts of South
India. The variability in pest susceptibility, like the variable expression
of the Cry1A proteins in Bt crops, will reduce the efficacy of Bt pest control.
However, using recombinant
CrylA proteins from bacteria to test for susceptibility in pests can be entirely
misleading (see below).
A study was carried out in the Institute of Plant Protection,
Chinese Academy of Agricultural Sciences in Beijing on two Bt cotton varieties:
GK19, with a Cry1Ac/Cry1Ab fused gene, developed by the Biotechnology Research
Institute of Chinese Academy of Agricultural Sciences, and BG1560, with a
Cry1Ac gene, supplied by Monsanto . The test site was in Tianmen County,
Hubei Province, an intensive planting area in the middle of the Yantze River
valley. The results showed that the toxin content in the Bt cotton varieties
changed significantly over time, depending on the part of the plant, the growth
stage and the variety. Generally, the toxin protein was expressed at high
levels during the early stages of growth, declined in mid-season, and rebounded
late in the season. In line with the study in India, the scientists found
that the toxin content in leaf, square, petal and stamens were generally much
high than those in the ovule and the boll. The researchers pointed out that
such variability in toxin expression could accelerate the development of pest
resistance to the toxin.
Scientists at the Southern Insect Management Research Unit of the United
States Department of Agriculture (USDA) studied both Bt maize hybrids expressing
Cry1Ab (such as event MON810) and Bt cotton varieties expressing Cry1Ac (such
as event MON531) .
They found that Cry1Ab was variable depending on location
in the same leaf as well as between leaves at different stage of growth. The
tips of maize leaf at the V7 stage had a higher concentration compared with
the middle section of the leaf, and the middle section of the V9 leaf had
the lowest concentration. Also, the green tissues richest in chlorophyll had
the highest toxin levels, the yellow-green tissues with reduce chlorophyll
had less, and the white-yellow tissues poorest in chlorophyll had the least.
The weight of fall armyworm larvae measured at day 5 of feeding showed a decrease
that was significantly correlated with the amount of toxin present in the
plant material, while there was 100 percent mortality in the southwestern
corn borer larvae regardless of the level of toxin in the plant tissues.
In the Bt cotton, the level
of CrylAc was significantly lower in boll tips where flowers had remained
attached, compared with normal boll tips. Boll tips where the flowers remained
attached are often the sites at which corn earworms, Helicopverpa zea (Boddie) penetrate Bt cotton bolls. In both
Bt maize and Bt cotton, tissues that had low chlorophyll content also had
reduced Cry1A proteins.
The US Environment Protection Agency recommends planting a certain percent
of crop area with non-Bt varieties to serve as ‘refuge’, in order to ensure
that enough susceptible insects are produced to limit the evolution of resistance.
An important requirement for the refuge strategy to work effectively is a high
level of expression of the toxin, so heterozygous insects (those with one copy
of resistance gene) will fail to survive to reproduce. Thus, any reduction from
high toxin levels will compromise the refuge strategy and the effectiveness
of Cry1A proteins in pest control.
Researchers at the University of Arizona Tucson and the Arizona Cotton Research
and Protection Council, Phoenix had found a “surprisingly high” frequency (0.16)
of the Cry1Ac resistance gene in field populations of the pink bollworm in Arizona
in 1997, which did not appear to increase further as expected in 1998 or 1999
. However, the tests were done with the recombinant Cry1Ac protein produced
in the bacterium, Pseudomonas fluroescens, and not from the Bt cotton
plant, and could be giving entirely misleading results on the evolution of resistance
in the field (“No Bt resistance?” SiS20)
Bt resistance in Australia
A population of the Australian cotton bollworm, Helicoverpa armigera – the most important
agricultural pest in Australia as well as China, India and Africa
- has developed resistance to Cry1Ac at 275-times the level that would have
killed the non-resistant insect . Some 70 percent of the resistant larvae
were able to survive on Bt cotton expressing Cry1Ac (Ingard). The resistance
is inherited as an autosomal semi-dominant trait (the heterozygote with one
copy of the resistance gene is half as resistant as the homozygotes with two
copies of the resistance gene).
Bt cotton varieties expressing Cry1Ac (Ingard) have been grown in Australia
to control the cotton bollworm since 1996, and a new variety containing both
Cry1Ac and Cry2Ab was commercially released in late 2003. Resistance monitoring
in Australia and China had suggested that pest susceptibility to Cry1Ac was
declining in the field. In 2001, a strain of cotton bollworm was isolated from
the survivors in the New South Wales and Queensland monitoring programme that
appeared to be resistant to Cry1Ac. The researchers have now confirmed the findings,
and attributed the high level of resistance to a 3- to 12-fold over-expression
of an enzyme, serine protease, which binds avidly to Cry1Ac toxin, preventing
it from acting, and possibly, detoxifying it by breaking it down.
Canadian scientists find yield and economic disadvantage in Bt maize
Researchers at the Eastern Cereal and Oilseed Research Centre, Agriculture
and Agri-Food Canada, Ottawa, Ontario, carried out a field experiment
over three years to compare commercial corn hybrids with their corresponding
Bt-hybrids belonging to the Monsanto and Syngenta . They found that some
of the Bt hybrids took 2-3 additional days to reach silking and maturity and
produced a similar or up to 12 percent lower grain yields, with 3-5 percent
higher grain moisture content at maturity in comparisons with their non-Bt
counterparts. Higher grain moisture content increases drying cost. Bt hybrid
seeds also have a $25-30 premium per ha.
The economic disadvantages are dwarfed in comparison with impacts on biodiversity
and human and animal health that have been known for years, however (see below:
also“Bt risks negligible” SiS
Bt maize more woody
It has been known for some time that genetic modification
is full of pitfalls, among which are many unintended effects. A paper published
in 2001  reported that the content of lignin (woody substances) was high
by 33 to 97 percent in the Bt maize varieties tested: Bt11, Bt176 and Mon810.
Now, researchers at environmental and agricultural institutes in Leipzig,
Aachen and Muncheberg, Germany, and the University of Waterloo in Ontario,
Canada, have confirmed increases in lignin in two Bt maize lines, Novelis
(event MON00810-6, from Monsanto) and Valmont (event SYN-EV176-9, from Syngenta),
compared with their respective isogenic varieties, Nobilis and Prelude, all
grown under identical conditions . The increases in lignin are more modest,
and are restricted to the stems of the plants: Novelis by 28 percent over
Nobilis, and Valmont by 18 percent over Prelude.
Increase in lignin content will impact on the digestibility of the plant for
livestock, it also decreases the rate at which the plant material break down,
affecting nutrient recycling, the soil microbial community, and soil carbon
Intriguingly, an earlier
report has also found increased lignin in Monsanto’s Roundup Ready soya, genetically
modified to be tolerant to the herbicide Roundup , which caused the stem
to split open in hot climate and crop losses of up to 40 percent.
These results suggest that
genetic modification per se
may be increasing lignin content, perhaps as a response to metabolic stress
from the high levels of transgene expression driven by aggressive viral promoters.
Impacts on biodiversity and health
Bt toxins are known to harm beneficial/endangered insect
species and soil decomposers :
- Pollen from Bt-maize was lethal to the larvae of the monarch butterfly.
- Increased mortality of lacewing larvae fed on artificial diet containing
Bt-maize or on corn-borer larvae that had eaten Bt-corn.
- Bt sprays used to reduce caterpillars in forests led to fewer black-throated
blue warbler nests.
- A parasite of corn-borers, Macrocentris cingulum, was found to be
reduced in Bt-cornfields compared with non-Bt corn fields.
- One preparation of Bt (var. tenebrionis), reported to be specific
for Coleoptera, caused significant mortality in domestic bees.
- Soil-dwelling collembola, Folsomia candida, an important decomposer,
suffered significant mortality from transgenic maize with Cry1Ab.
- Bt not only remains in the soil with Bt-plant debris, it is actively exuded
from the plant roots where it binds to soil particles and persists for 180
days or more, so its effects on soil decomposers and other beneficial arthropods
may be extensive.
Bt-toxins are actual and potential allergens for human beings. Field workers
exposed to Bt spray experienced allergic skin sensitization and induction of
IgE and IgG antibodies to the spray . Recombinant Cry1Ac protoxin was found
to be a potent mucosal immunogen, as potent as cholera toxin . A Bt strain
that caused severe human necrosis (tissue death) killed mice infected through
the nose within 8 hours, from clinical toxic-shock syndrome . Both Bt protein
and Bt-potato harmed mice in feeding experiments . All Bt-toxins along with
many other transgenic proteins exhibit similarities to known allergens and are
hence suspected allergens until proven otherwise (“Are transgenic proteins allergenic?”
SiS 25) [17-19].
Recently, much publicity
has been given to a report from scientists in Portugal published
in the house journal of the American Academy of Allergy, Asthma and Immunology, because it claimed
“lack of allergenicity of transgenic maize and soya samples” .
A careful reading of the
report reveals, however, that the researchers had no evidence that the small
number of subjects they tested have ever been exposed to transgenic maize
and soya. They wrote: “Bearing in mind that since 1998 all the GM products
under testing were approved for commercialisation in the European Union..,
we assumed that consumption of maize and soya
food-derived products implied a consumption of GM soya and maize.” (emphasis
added). Moreover, the tests performed were limited to skin pricks and IgE
antibodies, both known to be limited in reliability . Most of all, there
are many allergies that do not involve IgE antibodies .
Nevertheless, the researchers
stated, “In this study we did not obtain any differential positive results,
which allows us to conclude that the transgenic
products under testing seem to be safe regarding their allergenic potential.”